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Alexa Fluor® 594 AffiniPure F(ab')₂ Fragment Donkey Anti-Mouse IgG (H+L)

AlexaFluor®594 AffiniPure F(ab'')2片段驴抗小鼠IgG(H + L)

Company: Jackson ImmunoResearch
Catalog#: 715-095-166
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TRIPLE (Insulin, Glucagon and EGFP) Immunofluorescence Staining Protocol in Pancreas
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2014-03-05
[Abstract]  This protocol aims to introduce methods for immunostaining two endogenous proteins insulin and glucagon and one exogenous transgene driven EGFP in mouse pancreatic islet. The immunostaining results of insulin and glucagon indirectly tell functionality of pancreatic beta cells and alpha cells respectively. Furthermore, the protocol provides immunostaining steps for the third protein which can be applicable to any other endogenous proteins with a specific antibody generated in mouse. [摘要]  该协议旨在介绍在小鼠胰岛中免疫染色两种内源性蛋白质胰岛素和胰高血糖素和一种外源性转基因驱动的EGFP的方法。 胰岛素和胰高血糖素的免疫染色结果分别间接地指示胰腺β细胞和α细胞的功能。 此外,该方案提供了第三种蛋白质的免疫染色步骤,其可以应用于任何其他内源性蛋白质与在小鼠中产生的特异性抗体。

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