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Living Colors® A.v. Monoclonal Antibody (JL-8)

生活色彩® A.v.单克隆抗体(JL-8)

Company: Takara Bio
Catalog#: 632381
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Experimental Liver Fibrosis and Intrasplenic Transplantation of CD45+ Bone Marrow Cells
Author:
Date:
2016-10-20
[Abstract]  Liver fibrosis results from the excessive collagen deposition (collagen scar) by activated hepatic stellate cells (HpSCs), leading to the inhibition of normal liver regeneration and function. Fibrogenesis is a complex mechanism involving both the synthesis and degradation of matrix proteins by different cell types, mainly macrophages in the liver. Carbon tetrachloride-induced fibrosis (CCl4) and cirrhosis is one of the oldest, simplest and probably the most widely used toxin-based experimental model for the induction of fibrosis. Here we have explained experimental animal model of liver fibrosis using CCl4, injecting twice a week for a period of 8 weeks. In these fibrotic mice, bone marrow (BM) derived CD45+ cells were transplanted via intrasplenic route ... [摘要]  Liver fibrosis results from the excessive collagen deposition (collagen scar) by activated hepatic stellate cells (HpSCs), leading to the inhibition of normal liver regeneration and function. Fibrogenesis is a complex mechanism involving both the synthesis and degradation of matrix proteins by different cell types, mainly macrophages in the liver. Carbon tetrachloride-induced fibrosis (CCl4) and cirrhosis is one of the oldest, simplest and probably the most widely used toxin-based experimental model for the induction of fibrosis. Here we have explained experimental animal model of ...

TRIPLE (Insulin, Glucagon and EGFP) Immunofluorescence Staining Protocol in Pancreas
Author:
Date:
2014-03-05
[Abstract]  This protocol aims to introduce methods for immunostaining two endogenous proteins insulin and glucagon and one exogenous transgene driven EGFP in mouse pancreatic islet. The immunostaining results of insulin and glucagon indirectly tell functionality of pancreatic beta cells and alpha cells respectively. Furthermore, the protocol provides immunostaining steps for the third protein which can be applicable to any other endogenous proteins with a specific antibody generated in mouse. [摘要]  该协议旨在介绍在小鼠胰岛中免疫染色两种内源性蛋白质胰岛素和胰高血糖素和一种外源性转基因驱动的EGFP的方法。 胰岛素和胰高血糖素的免疫染色结果分别间接地指示胰腺β细胞和α细胞的功能。 此外,该方案提供了第三种蛋白质的免疫染色步骤,其可以应用于任何其他内源性蛋白质与在小鼠中产生的特异性抗体。

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