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Author:
Date:
2015-03-05
[Abstract] Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern and western blotting, genome-wide microarray analysis or qRT-PCR. Here, we describe a method for the isolation and quantification of high-quality polysome-bound mRNA complexes from small quantities of liquid-nitrogen-frozen solid tissue samples of rice shoots/roots. The mRNA obtained can be further analyzed by methods that evaluate polysomal mRNA abundance at the individual transcript or global level.
[摘要] Polysome谱分析是翻译控制研究中经常执行的任务，不仅能够直接监测翻译的效率，而且可以很容易地扩展到广泛的下游应用，如Northern和western印迹，全基因组微阵列分析或qRT- PCR。 在这里，我们描述了一种从少量液氮冷冻固体组织样本的水稻芽/根中分离和定量高质量多糖结合mRNA复合物的方法。 获得的mRNA可以通过在单个转录物或全局水平评估多核糖体mRNA丰度的方法进一步分析。
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