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Fetal calf serum (FCS)

胎牛血清(FCS)

Company: Greiner Bio One International
Catalog#: 758072
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Monocyte-MSC Co-cultures
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Date:
2015-01-20
[Abstract]  To assess the effect of multipotent stromal cells (MSC) on monocytes, 3-day cultures were performed of freshly isolated monocytes in MSC-conditioned medium (CM). As a control condition, monocytes were stimulated with low dose macrophage colony-stimulating factor (M-CSF). Monocytes were isolated from peripheral blood mononuclear cell (PBMC) populations by magnetic activated cell sorting (MACS) using CD14 microbeads. [摘要]  为了评估多潜能基质细胞(MSC)对单核细胞的作用,在MSC条件培养基(CM)中对新鲜分离的单核细胞进行3天培养。 作为对照条件,用低剂量巨噬细胞集落刺激因子(M-CSF)刺激单核细胞。 通过磁激活细胞分选(MACS)使用CD14微珠从外周血单核细胞(PBMC)群体中分离单核细胞。

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