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Author:
Date:
2014-11-20
[Abstract] Lipopolysaccharide is the major constituent of the outer membrane of gram-negative bacteria and, once released from the bacterial surface into the bloodstream, is a potent activator of the host immune system, which can lead to septic shock. LPS has a hydrophilic region consisting of a repeating oligosaccharide that is strain-specific (O-antigen) and a core polysaccharide, which is covalently linked to a hydrophobic lipid moiety (lipid A). Lipid A is the most conserved part and is responsible for the toxicity of LPS. Therefore, finding molecules able to bind to this region and neutralize LPS toxicity is of relevant interest as it may provide new therapies to prevent septic shock (Chen et al., 2006). Several proteins and peptides were reported to bind LPS and alter its toxicity ...
[摘要] 脂多糖是革兰氏阴性细菌外膜的主要成分，一旦从细菌表面释放到血液中，是宿主免疫系统的有效激活剂，可导致败血性休克。 LPS具有由与疏水性脂质部分（脂质A）共价连接的作为菌株特异性（O-抗原）的重复寡糖和核心多糖组成的亲水性区域。脂质A是最保守的部分，负责LPS的毒性。因此，寻找能够结合该区域并中和LPS毒性的分子是有兴趣的，因为它可能提供新的治疗方法来预防败血性休克（Chen et al。，2006）。报道了几种蛋白质和肽结合LPS并改变其对还原和甚至增强的毒性（Brandenburg等，1998），例如血清白蛋白（Ohno和Morrison，1989），脂多糖结合蛋白（LBP）（de Haas等人，1999），酪蛋白（López-Expósito等，2008），溶菌酶，抗生素多粘菌素B和抗菌肽（Chen等，2006）。虽然这些蛋白质中的一些是中性的，甚至阴离子/酸性（pI <7）（jang et="" al。，2009），由于lps的两亲性结构和脂质a上带负电荷的磷酸酯基团的存在是最重要的因素被认为与lps最佳结合的是阳离子/碱性（pi=""> ...
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