| DNA PCR Assays for Igh Rearrangement
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Author:
Date:
2014-02-20
[Abstract] This protocol is used for the detection of immunoglobulin heavy (H) chain rearrangements. This PCR-based assay enables detection of DH-JH recombination in cultured hematopoietic cells (Schlissel et al., 1991; Satoh et al., 2013) [e.g. ES-derived cells (Satoh et al., 2013)].
[摘要] 该方案用于检测免疫球蛋白重链（H）链重排。 这种基于PCR的测定使得能够在培养的造血细胞中检测D H H -J H亚基重组（Schlissel等人，1991; Satoh et al。，2013）[例如 ES衍生细胞（Satoh et al。，2013）]。
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| Cell Culture Mycoplasma Detection by PCR
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Author:
Date:
2012-04-20
[Abstract] DNA was extracted from the supernatant of each sample. After PCR-amplification of mycoplasma DNA, detection was performed by gel electrophresis. The PCR primers were designed to cover the consensus sequences that can detect all types of mycoplasma species.
[摘要] 从每个样品的上清液中提取DNA。 在支原体DNA的PCR扩增后，通过凝胶电泳进行检测。 设计PCR引物以覆盖可以检测所有类型的支原体物种的共有序列。
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