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Author:
Date:
2014-10-20
[Abstract] In cigarette smoke–induced and inflammation-associated lung cancer development, cigarette smoke extract (CSE) activates tumor necrosis factor-alpha (TNF-α) secretion from macrophages. TNF-α converting enzyme (TACE), also known as α-Secretase or ADAM17 (A Disintegrin and Metalloprotease), is a member of the ADAM family of metalloproteases. TACE mediated ectodomain shedding leads to the conversion of the inactive TNF-α precursor into the active mature pro-inflammatory cytokine. The SensoLyte 520 TACE (α-Secretase) Activity Assay Kit was used to detect TACE activity in CSE-activated macrophages. This assay is reliable, reproducible and easy to carry out in 96 well plate format.
[摘要] 在香烟烟雾诱导和炎症相关的肺癌发展中，香烟烟雾提取物（CSE）激活巨噬细胞的肿瘤坏死因子-α（TNF-α）分泌。 TNF-α转化酶（TACE），也称为α-分泌酶或ADAM17（解聚素和金属蛋白酶），是金属蛋白酶的ADAM家族的成员。 TACE介导的胞外结构域脱落导致无活性的TNF-α前体转化为活性成熟促炎细胞因子。 SensoLyte 520 TACE（α-分泌酶）活性测定试剂盒用于检测CSE活化的巨噬细胞中的TACE活性。 该测定法是可靠的，可重复的并且容易在96孔板形式中进行。
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