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Author:
Date:
2014-10-05
[Abstract] Like many woody plant species, walnut (Juglans regia) can be difficult to genetically transform and regenerate. However, somatic embryos have been used successfully for over two decades as a target tissue for transformation and regeneration of transgenic walnut plants. Walnut somatic embryos, initiated originally from developing zygotic embryos or anther tissue, will proliferate numerous secondary embryos from single cells in the epidermal layer. These single cells in intact somatic embryos can be efficiently transformed by Agrobacterium tumefaciens (A. tumefaciens). This gene transfer system is most efficient when Agrobacterium binary vector plasmids contain a scorable maker gene (e.g. uidA) and a selectable marker gene (e.g. nptII). This ...
[摘要] 像许多木本植物物种一样，核桃（ Juglans regia ）可能难以进行遗传转化和再生。然而，体细胞胚已经成功使用超过二十年作为转基因核桃植物的转化和再生的靶组织。核桃体细胞胚，最初从发育的合子胚或花药组织开始，将从表皮层中的单个细胞增殖许多次级胚。在完整的体细胞胚中的这些单细胞可以通过根癌土壤杆菌（根瘤土壤杆菌）有效地转化（ tumefaciens ）。当农杆菌二元载体质粒含有可打分的基因（例如uidA）和选择性标记基因（例如nptII）时，该基因转移系统是最有效的。该系统应适用于从单个农杆菌感受细胞经历重复胚胎发生的任何作物。在这里我们详细描述转化体细胞胚胎的方法，使这种技术可以应用于核桃和其他木本植物物种。
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