|
Author:
Date:
2013-09-05
[Abstract] Targeted photothrombosis is a method to occlude individual arterioles and venules that lie on the surface of the cerebral cortex. It has been used to study collateral flow patterns within the pial vascular network following occlusion of single surface vessels (Schaffer et al., 2006; Blinder et al., 2010; Nguyen et al., 2011), as well as to generate localized ischemic strokes following occlusion of single penetrating vessels (Nishimura et al., 2007; Drew et al., 2010; Shih et al., 2013). The intravascular clot is formed by irradiation of a target vessel with a focused green laser after injection of a circulating photosensitizing agent, Rose Bengal (Watson et al., 1985). We briefly describe modifications of custom-designed and ...
[摘要] 目标光血栓形成是阻塞位于大脑皮质表面上的个体小动脉和小静脉的方法。它已经用于在封闭单表面血管后研究在Pial血管网内的侧支血流模式（Schaffer等人，2006; Blinder等人，2010; Nguyen 2011），以及在单一穿透血管闭塞后产生局部缺血性中风（Nishimura et al。，2007; Drew et al。 ，2010; Shih et al。，2013）。在注射循环的光敏剂，玫瑰红（Watson等人，1985）后，通过用聚焦的绿色激光器照射目标血管形成血管内凝块。我们简要介绍了定制设计和商业双光子成像系统的修改，引入绿色激光用于光血栓形成。我们进一步提供如何闭塞麻醉鼠标的体感皮层内的单个穿透小动脉的说明。
|