Blocking reagent
Company: Roche Diagnostics
Catalog#: 11096176001
Product evaluation:
Detection of ALT Associated Promyelocytic Leukemia Nuclear Bodies (APBs) by Immunofluorescence-FISH (IF-FISH)
Author:
Date:
2014-12-05
[Abstract]  The activation of functions that counteract the physiological shortening of telomeres in rapidly proliferating cell is prerequisite for the progression of cancer cells to full malignancy (Collado et al., 2007). In most human cancers, the length of telomere is maintained through up-regulation of telomerase whereas a telomerase-independent pathway, termed Alternative Lengthening of Telomeres (ALT) is active in about 10-15% of cancers (Johnson and Broccoli, 2007; Heaphy et al., ...

ImmunoFISH for Adherent Cultured Mammalian Cells
Author:
Date:
2013-12-20
[Abstract]  This protocol is optimized for immunoFISH staining of adherent cultured mammalian cells. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) and FISH against telomeric DNA.

ImmunoFISH for Mice and Baboons Frozen Sections
Author:
Date:
2013-12-20
[Abstract]  This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.

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