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Author:
Date:
2013-08-05
[Abstract] The protocol describes preparation of young developing maize kernels for microscopical analysis of nuclei in tissue sections. The fixative FAA (formaldehyde, acetic acid and ethanol) is suitable for preservation of nuclear morphology and allows for quantitative staining of DNA with Schiff reagent in Feulgen staining. The fixation and embedding protocol may be used also for various other histology staining procedures, but care must be taken as the cytoplasm usually shrinks a bit using this procedure. The protocol was used for analysis of seed development of various maize lines, mutants and maize relatives (Vilhar et al., 2002; Kladnik et al., 2006; Dermastia et al., 2009; Bernardi et al., 2012).
[摘要] 该协议描述了用于组织切片中核的显微镜分析的年轻发育的玉米籽粒的制备。 固定剂FAA（甲醛，乙酸和乙醇）适合保存核形态，并允许在Feulgen染色中用希夫试剂定量染色DNA。 固定和包埋方案也可以用于各种其他组织学染色程序，但是必须小心，因为细胞质通常使用该程序略微收缩。 该方案用于分析各种玉米品系，突变体和玉米亲缘种的种子发育（Vilhar等人，2002; Kladnik等人，2006; Dermastia >等人，2009; Bernardi等人，2012）。
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