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Pipette tips 10/20 μl

0.1-10 / 20ul XL TipOne过滤嘴,无菌

Company: USA Scientific
Catalog#: 1120-3810
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Total RNA Extraction from Dinoflagellate Symbiodinium Cells
Author:
Date:
2018-06-05
[Abstract]  Dinoflagellates are unicellular algae that can have photosynthetic or nonphotosynthetic lifestyles. Dinoflagellates in the genus Symbiodinium can enter endosymbiotic associations with corals, providing the metabolic basis for the highly productive and biologically diverse coral-reef ecosystems (Hoegh-Guldberg, 1999), as well as with other cnidarians, including sea anemones and jellyfish, and non-cnidarian hosts (Trench, 1993; Lobban et al., 2002; Mordret et al., 2016).

Here, I describe a protocol for isolating total RNA from Symbiodinium cells.
[摘要]  鞭毛藻是单细胞藻类,可以有光合或非光合生活方式。 Symbiodinium属中的甲藻类可以与珊瑚进入内共生关系,为高生产力和生物多样性珊瑚礁生态系统提供代谢基础(Hoegh-Guldberg,1999),以及其他的cnidarians,包括 海葵和海蜇,以及非刺猬寄主(Trench,1993; Lobban等人,2002; Mordret等人,2016)。

在这里,我描述了从Symbiodinium细胞中分离总RNA的方案。

Single-cell Gene Expression Profiling of Mouse Stem Cells With Fluidigm BiomarkTM Dynamic Array
Author:
Date:
2013-05-05
[Abstract]  This protocol describes how to use Fluidigm BiomarkTM 96.96 dynamic arrays for high-throughput expression profiling from single mouse stem cells, assaying up to 96 independent samples with up to 96 quantitative PCR (qPCR) probes (equivalent to 9,216 reactions) in a single experiment. This Dynamic Array contains a network of microfluidic channels, chambers and valves that automatically assemble all these individual PCR reactions. Single-cell profiling can provide definitive evidence of stem cell heterogeneity. Modifications are most likely needed if users intend to use BiomarkTM 48.48 Dynamic array or experimenter-designed primers in conjunction with DNA-binding dyes such as EvaGreen (Biotium 31000). [摘要]  该方案描述了如何使用Fluidigm Biomark?sup?9696动态阵列从单个小鼠干细胞进行高通量表达谱分析,使用多达96个定量PCR(qPCR)探针测定多达96个独立样品(相当于 9,216个反应)。 该动态阵列包含微流体通道,腔室和阀门的网络,其自动组装所有这些单独的PCR反应。 单细胞谱分析可以提供干细胞异质性的明确证据。 如果用户打算使用Biomark TM 48.48动态阵列或实验设计的引物与DNA结合染料如EvaGreen(Biotium 31000)结合,则很可能需要进行修饰。

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