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Author:
Date:
2013-05-05
[Abstract] This protocol describes how to use Fluidigm BiomarkTM 96.96 dynamic arrays for high-throughput expression profiling from single mouse stem cells, assaying up to 96 independent samples with up to 96 quantitative PCR (qPCR) probes (equivalent to 9,216 reactions) in a single experiment. This Dynamic Array contains a network of microfluidic channels, chambers and valves that automatically assemble all these individual PCR reactions. Single-cell profiling can provide definitive evidence of stem cell heterogeneity. Modifications are most likely needed if users intend to use BiomarkTM 48.48 Dynamic array or experimenter-designed primers in conjunction with DNA-binding dyes such as EvaGreen (Biotium 31000).
[摘要] 该方案描述了如何使用Fluidigm Biomark？sup？9696动态阵列从单个小鼠干细胞进行高通量表达谱分析，使用多达96个定量PCR（qPCR）探针测定多达96个独立样品（相当于 9,216个反应）。 该动态阵列包含微流体通道，腔室和阀门的网络，其自动组装所有这些单独的PCR反应。 单细胞谱分析可以提供干细胞异质性的明确证据。 如果用户打算使用Biomark TM 48.48动态阵列或实验设计的引物与DNA结合染料如EvaGreen（Biotium 31000）结合，则很可能需要进行修饰。
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