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Author:
Date:
2014-03-20
[Abstract] In the past years, a subset of regulatory T cells (Tregs) expressing CD4, CD25 and the transcription factor FoxP3 has gained considerable attention as key regulators of T-cell tolerance and homeostasis (Sakaguchi, 2004). This population of T cells is specifically engaged in the maintenance of immune self-tolerance and the control of aberrant immune responses to foreign antigens. Remarkably, regulatory T cells have been implicated in tumor cell evasion of immune responses (Curiel et al., 2004; Zou, 2006) by suppressing T cell mediated antitumor immunity. The study of the signals that promote the differentiation of this suppressive population in the tumor microenvironment has become a central issue. Here we described a detailed method to in vitro differentiate Tregs using ...
[摘要] 在过去的几年中，表达CD4，CD25和转录因子FoxP3的调节性T细胞（Treg）的一个子集已经作为T细胞耐受性和体内平衡的关键调节剂获得了相当大的关注（Sakaguchi，2004）。 这种T细胞群特别参与维持免疫自身耐受性和控制对外来抗原的异常免疫应答。 值得注意的是，调节性T细胞通过抑制T细胞介导的抗肿瘤免疫参与了肿瘤细胞逃避免疫应答（Curiel等人，2004; Zou，2006）。 促进这种抑制群体在肿瘤微环境中的分化的信号的研究已经成为一个中心问题。 在这里，我们描述了使用来自小鼠天然T细胞的肿瘤细胞条件培养基来体外鉴别Treg的详细方法，并且基于它们的特异性标记物来鉴定它们（Dalotto-Moreno等人， >，2013）。
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