{{'Search' | translate}}
 

4’, 6-diamidine-2-phenylindole dihydrochloride (DAPI)

4'',6-二脒-2-苯基吲哚二盐酸盐(DAPI)

Company: Boehringer Mannheim
Catalog#: 236 276
Bio-protocol()
Company-protocol()
Other protocol()

Immunofluorescence Assay for S Phase Entry Using BrdU Incorporation
Author:
Date:
2012-02-05
[Abstract]  Bromodeoxyuridine (BrdU) is thymidine analogue and can incorporated into the newly synthezised DNA of S-phase cells, the antibody specific for BrdU can then be used to detect the incorporated BrdU, thus indicating cells that were actively replicating their DNA and estimating for the percentage of cells in S-phase. The immunocytochemical detection of BrdU incorporated into DNA is a powerful tool to study the cytokinetics of normal and tumor cells. In vitro or in vivo labeling of tumor cells with BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. [摘要]  溴脱氧尿苷(BrdU)是胸苷类似物并且可以并入S期细胞的新合成的DNA中,然后可以使用对BrdU特异性的抗体来检测掺入的BrdU,从而指示活跃地复制其DNA的细胞, 的S期细胞。 掺入DNA的BrdU的免疫细胞化学检测是研究正常和肿瘤细胞的细胞因子的有力工具。 在体外或体内用BrdU标记肿瘤细胞,随后用特异性抗BrdU单克隆抗体检测掺入的BrdU是一种准确和全面的方法, DNA合成。

Comments