|
Author:
Date:
2013-04-20
[Abstract] Formation of viral particles and packaging of genomic retroviral RNA into these particles are important steps in the late phase of the viral replication cycle. The efficiency of the incorporation of viral or cellular RNAs into viral particles can be studied using a quantitative Reverse Transcriptase-PCR (RT-qPCR)-based approach. After isolation of cytoplasmic RNA from either infected or transfected cells and extraction of virus particle-associated RNA, specific RNA levels present in both fractions are determined. The ratio of virion-associated and cytoplasmic RNA defines the encapsidation efficiency (Brandt et al., 2007; Blissenbach et al., 2010; Grewe et al., 2012).
[摘要] 病毒颗粒的形成和基因组逆转录病毒RNA包装到这些颗粒中是病毒复制周期晚期的重要步骤。 可以使用定量逆转录酶-PCR（RT-qPCR）为基础的方法研究将病毒或细胞RNA掺入病毒颗粒中的效率。 在从感染的或转染的细胞分离细胞质RNA并提取病毒颗粒相关的RNA后，测定两种级分中存在的特异性RNA水平。 病毒体相关的和细胞质RNA的比率定义了衣壳化效率（Brandt等人，2007; Blissenbach等人，2010; Grewe等人，/em>，2012）。
|