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Author:
Date:
2014-01-20
[Abstract] Methyltransferase (MTase) transfers a methyl group (-CH3) from the donor S-adenosyl-L-methionine (AdoMet or SAM) to biologically active molecules such as hormones, neurotransmitters, lipids, proteins and nucleic acids. The addition of a methyl group causes a change in the physicochemical properties of the molecules. The mRNA cap structure is essential for cell and virus. Guanine-N7-methyltransferase (N7-MTase) methylates the GpppN cap at the N7 position of guanine, resulting in cap-0 structure (m7GpppN), and Ribose 2'-O-MTase further methylates the first nucleotide of higher eukaryotic cellular and viral mRNAs at the ribose 2'-OH position to form cap-1 (m7GpppNm) structures. Here, we describe a biochemical assay to detect the activities of mRNA capping MTases.
[摘要] 甲基转移酶（MTase）将甲基（-CH 3）从供体S-腺苷-L-甲硫氨酸（AdoMet或SAM）转移到生物活性分子例如激素，神经递质，脂质，蛋白质和核酸。 加入甲基引起分子的物理化学性质的改变。 mRNA帽结构对于细胞和病毒是必需的。 鸟嘌呤-N7-甲基转移酶（N7-MTase）甲基化在鸟嘌呤的N7位置的GpppN帽，导致cap-0结构（m7GpppN），核糖2'-O-MTase进一步甲基化高等真核细胞和病毒的第一个核苷酸 mRNA在核糖2'-OH位置形成cap-1（m7GpppNm）结构。 在这里，我们描述一种生物化学测定检测mRNA上限MTases的活动。
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