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Protein A Agarose/Salmon Sperm DNA, 2.5 mL

蛋白A琼脂糖/鲑精DNA,2.5mL

Company: EMD Millipore
Catalog#: 16-157
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ChIP Assay for Cell Culture
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Date:
2011-01-20
[Abstract]  Chromatin immunoprecipitation (ChIP) is a method used to determine the location of DNA binding sites on the genome for a particular protein of interest. Following crosslinking, cells are lysed and the DNA is broken into pieces 0.2-1.0 kb in length by sonication. At this point immunoprecipitation is performed resulting in the purification of protein–DNA complexes. The identity and quantity of the isolated DNA fragments can then be determined by PCR. This protocol describes how to perform a ChIP experiment and can be applied to different types of cell culture. [摘要]  染色质免疫沉淀(ChIP)是用于确定特定目标蛋白质的基因组上的DNA结合位点的位置的方法。 交联后,裂解细胞,通过超声处理将DNA断裂成长度为0.2-1.0kb的片段。 此时进行免疫沉淀,从而纯化蛋白质-DNA复合物。 然后可以通过PCR测定分离的DNA片段的身份和数量。 该协议描述了如何进行ChIP实验,并可以应用于不同类型的细胞培养。

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