| Intestinal Differentiation of Human ESCs
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Author:
Date:
2013-12-20
[Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial cells. Here, we describe a protocol, in which differentiated cell types of the intestine are derived from human ESCs. In this protocol, we describe a protocol to differentiate mouse ES cells into ...
[摘要] ES细胞（ESC）是多能性的，并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反，胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞：肠细胞，杯状细胞，肠内分泌细胞和Paneth细胞。肠干细胞（ISCs）和祖细胞驻留在隐窝中，有力地增殖，并且作为分化的上皮细胞的来源。在这里，我们描述了一种协议，其中分化的细胞类型的肠来自人类胚胎干细胞。在这个协议中，我们描述一个协议，通过与M15，小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂，激活Wnt信号通路，DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT处理产生来自人ESC的所有代表性细胞谱系，肠细胞，杯状细胞，肠内分泌细胞和paneth细胞。人ESC的方案主要与用于小鼠ESC的方案非常相似，具有一些修改。
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| Intestinal Differentiation of Mouse ESCs
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Author:
Date:
2013-12-20
[Abstract] ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial cells. Here, we describe a protocol, in which differentiated cell types of the intestine are derived from mouse ESCs. In this protocol, we describe a protocol to differentiate mouse ES cells into ...
[摘要] ES细胞（ESC）是多能性的，并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反，胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞：肠细胞，杯状细胞，肠内分泌细胞和Paneth细胞。肠干细胞（ISCs）和祖细胞驻留在隐窝中，有力地增殖，并且作为分化的上皮细胞的来源。在这里，我们描述一种协议，其中分化的细胞类型的肠来自小鼠胚胎干细胞。在这个协议中，我们描述一个协议，通过与M15，小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂，激活Wnt信号通路，DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT治疗产生所有代表性的细胞谱系，肠细胞，杯状细胞，肠内分泌细胞和paneth细胞，来源于小鼠ESC。
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| Mouse ESC Differentiation to Nkx2.1+ Lung and Thyroid Progenitors
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Author:
Date:
2012-11-20
[Abstract] The de novo derivation of lung progenitors from pluripotent stem cells provides the opportunity to model early lung development in vitro and allows easy access to cells for tissue engineering or basic cell biology studies. This detailed protocol allows the generation of lung and thyroid progenitors from mouse embryonic stem cell (ESC) or induced pluripotent stem cell (iPSC) lines. When used together with a published Nkx2.1-GFP knock-in ESC line, the protocol allows tracking and purification of lung and thyroid progenitors by sorting on the GFP reporter based on the induction of the earliest known marker of lung and thyroid cell fate, Nkx2.1. After sorting, a pure population of Nkx2.1+ cells can then be replated for further expansion, differentiation, and maturation in ...
[摘要] 来自多能干细胞的肺祖细胞的新发现提供了在体外模拟早期肺发育的机会，并且容易获得用于组织工程或基础细胞生物学研究的细胞。 该详细方案允许从小鼠胚胎干细胞（ESC）或诱导多能干细胞（iPSC）系生成肺和甲状腺祖细胞。 当与公布的Nkx2.1-GFP敲入ESC系一起使用时，该方案允许通过基于对最早已知的肺和甲状腺细胞命运的标记物的诱导在GFP报告基因上进行分选来跟踪和纯化肺和甲状腺祖细胞， Nkx2.1。 在分选后，然后可以在无血清条件下培养纯化的Nkx2.1 +细胞群，以进一步扩增，分化和成熟。
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