Genetic Analyzer

遗传分析仪

Company: Thermo Fisher Scientific

Catalog#: ABI PRISM^® 3100 Genetic Analyzer

Similar Protocol

Application of a Spacer-nick Gene-targeting Approach to Repair Disease-causing Mutations with Increased Safety

Ngoc Tung Tran, Mikhail Lebedin, Eric Danner, Ralf Kühn, Klaus Rajewsky and Van Trung Chu

Published online: 2023-04-20

Bio-protocol()

Company-protocol()

Other protocol()

CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae

Author:

Yu Sasano and Satoshi Harashima,

Date:

2017-05-20

[Abstract] Chromosome engineering is an important technology with applications in basic biology and biotechnology. Chromosome splitting technology called PCS (PCR-mediated Chromosome Splitting) has already been developed as a fundamental chromosome engineering technology in the budding yeast. However, the splitting efficiency of PCS technology is not high enough to achieve multiple splitting at a time. This protocol describes a procedure for achieving simultaneous and multiple chromosome splits in the budding yeast Saccharomyces cerevisiae by a new technology called CRISPR-PCS. At least four independent sites in the genome can be split by one transformation. Total time and labor for obtaining a multiple split yeast strain is drastically reduced when compared with conventional PCS ... [摘要] 染色体工程是应用于基础生物学和生物技术的重要技术。染色体分裂技术称为PCS（PCR介导的染色体分裂）已经被开发为发芽酵母中的基本染色体工程技术。然而，PCS技术的分割效率不够高，不能一次实现多次分割。该协议描述了通过称为CRISPR-PCS的新技术在芽状酵母酿酒酵母中实现同时和多个染色体分裂的过程。基因组中至少四个独立的位点可以通过一次转化来分裂。与常规PCS技术相比，获得多重分裂酵母菌株的总时间和劳动力大大降低。

背景能够快速有效地操纵多个遗传基因座或染色体区域的染色体工程技术变得越来越重要。这些技术为阐明染色体和基因组功能提供了有力的手段。此外，它可以用于通过创建广泛的遗传变体来繁殖有用的菌株。在芽殖酵母酿酒酵母（Saccharomyces ...

Identification of Natural Hybrids by SSR Markers in Mussaenda

Author:

Zhonglai Luo, Tingting Duan, Shuai Yuan, Shi Chen, Xiufeng Bai and Dianxiang Zhang,

Date:

2016-07-05

[Abstract] Detection of natural hybrids is of great significance for plant taxonomy, reproductive biology, and population genetic studies. Compared with methods depending on morphological characters, molecular markers provide reliable and much more accurate results. This protocol describes approaches employing microsatellite (SSR) markers to identify inter-specific hybrids in Mussaenda (Rubiaceae). [摘要] 自然杂交的检测对植物分类学，生殖生物学和群体遗传学研究具有重要意义。与依赖于形态特征的方法相比，分子标记提供可靠和更准确的结果。该协议描述了采用微卫星（SSR）标记来鉴定Mussaenda（茜草科）中的特异性杂交体的方法。

Comments

Genetic Analyzer

遗传分析仪

免责声明