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200mM‐L‐Glutamine Stock Solution

200mM-L-谷氨酰胺储备液

Company: NACALAI TESQUE
Catalog#: 16948-04
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Intestinal Differentiation of Human ESCs
Author:
Date:
2013-12-20
[Abstract]  ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial cells. Here, we describe a protocol, in which differentiated cell types of the intestine are derived from human ESCs. In this protocol, we describe a protocol to differentiate mouse ES cells into ... [摘要]  ES细胞(ESC)是多能性的,并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反,胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞:肠细胞,杯状细胞,肠内分泌细胞和Paneth细胞。肠干细胞(ISCs)和祖细胞驻留在隐窝中,有力地增殖,并且作为分化的上皮细胞的来源。在这里,我们描述了一种协议,其中分化的细胞类型的肠来自人类胚胎干细胞。在这个协议中,我们描述一个协议,通过与M15,小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂,激活Wnt信号通路,DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT处理产生来自人ESC的所有代表性细胞谱系,肠细胞,杯状细胞,肠内分泌细胞和paneth细胞。人ESC的方案主要与用于小鼠ESC的方案非常相似,具有一些修改。

Intestinal Differentiation of Mouse ESCs
Author:
Date:
2013-12-20
[Abstract]  ES cells (ESCs) are pluripotent and offer a good tool to study early embryonic development. Intestinal cells are derived from the definitive endoderm. In contrast to adult tissue stem cells, embryonic development and differentiation from ES cells have not been as well investigated in the intestine. There are four differentiated cell types of non-proliferative epithelial cells: enterocytes, goblet cells, enteroendocrine cells, and Paneth cells. Intestinal stem cells (ISCs) and progenitor cells reside in crypts, proliferate vigorously, and function as the source of differentiated epithelial cells. Here, we describe a protocol, in which differentiated cell types of the intestine are derived from mouse ESCs. In this protocol, we describe a protocol to differentiate mouse ES cells into ... [摘要]  ES细胞(ESC)是多能性的,并且提供了研究早期胚胎发育的良好工具。肠细胞来源于定形内胚层。与成体组织干细胞相反,胚胎发育和胚胎干细胞的分化在肠道中没有得到很好的研究。存在四种分化的细胞类型的非增殖性上皮细胞:肠细胞,杯状细胞,肠内分泌细胞和Paneth细胞。肠干细胞(ISCs)和祖细胞驻留在隐窝中,有力地增殖,并且作为分化的上皮细胞的来源。在这里,我们描述一种协议,其中分化的细胞类型的肠来自小鼠胚胎干细胞。在这个协议中,我们描述一个协议,通过与M15,小鼠中肾细胞系共同培养和用两种化合物治疗有效地将小鼠ES细胞分化为表达Cdx2的肠内胚层。使用的化学化合物是BIO和DAPT。 BIO是Gsk3抑制剂,激活Wnt信号通路,DAPT是抑制Notch信号通路的α-分泌酶抑制剂。 BIO和DAPT治疗产生所有代表性的细胞谱系,肠细胞,杯状细胞,肠内分泌细胞和paneth细胞,来源于小鼠ESC。

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