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Author:
Date:
2021-02-20
[Abstract] Sphingolipids are major structural components of endomembranes and have also been described as an intracellular second messenger involved in various biological functions in all eukaryotes and a few prokaryotes. Ceramides (Cer), the central molecules of sphingolipids, have been depicted in cell growth arrest, cell differentiation, and apoptosis. With the development of lipidomics, the identification of ceramides has been analyzed in many species, mostly in model insects. However, there is still a lack of research in non-model organisms. Here we describe a relatively simple and sensitive method for the extraction, identification, and quantification of ceramides in Hemiptera Insects (brown planthooper), followed by Ultra-Performance Liquid Chromatography coupled to tandem mass spectrometry ...
[摘要] [摘要]鞘脂类是endomembranes的主要结构部件和已经也被描述为参与所有真核生物和原核生物几多种生物学功能的细胞内第二信使。 神经酰胺（CER），鞘脂的中心的分子，已经在细胞生长停滞，细胞分化被描述，和细胞凋亡。随着脂质组学的发展，已经在许多物种中对神经酰胺的鉴定进行了分析，其中大多数是模型昆虫。但是，仍然缺乏对非模型生物的研究。在这里，我们描述了用于提取中相对简单和灵敏的方法N，鉴定，并在半翅目昆虫的神经酰胺的定量（棕色planthooper ），随后用超高效液相色谱-串联质谱联用（UPLC-MS / MS）。C18被用作用于对三联四极液体MAS定量检测和分析的分离柱小号光谱仪。在该方案中，采用标准曲线方法以根据可选的检测条件确认更准确的神经酰胺定量。
[背景]鞘脂是膜脂质在活体生物的第二大组和细胞结构，代谢和调节的许多方面发挥了重要作用（拉希里和Futerman，2007）。起初，人们认为鞘脂是结构相关分子的复杂家族，但越来越多的研究表明鞘脂参与了许多细胞过程（Mao和Obeid ，2008 ）。神经酰胺（CER）是必不可少的牵连生物活性脂质的各种细胞生物过程从细胞生长调控细胞死亡和衰老（Futerman和Hannun，2004 ; ...
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