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Author:
Date:
2016-05-20
[Abstract] Sphingosine 1-phosphate (S1P) is a lipid metabolite and signaling molecule involved in many different physiological processes including lymphocyte circulation, T cell differentiation, antigen presentation, and maintenance of the vascular endothelial barrier. S1P is a ligand of five different G protein-coupled cell surface receptors designated S1P1-5. It has also been described as an intracellular second messenger. Quantification of S1P in biological samples is therefore an important task to decipher its signaling capabilities in vivo under physiological and pathophysiological conditions in different body fluids and organs. In this protocol, quantification of S1P is performed by liquid chromatography coupled to triple-quadrupole mass spectrometry (LC-MS/MS).
[摘要] 鞘氨醇1-磷酸（S1P）是参与许多不同生理过程的脂质代谢物和信号分子，所述生理过程包括淋巴细胞循环，T细胞分化，抗原呈递和血管内皮屏障的维持。 S1P是称为S1P 1-5的五种不同的G蛋白偶联的细胞表面受体的配体。 它也被描述为细胞内第二信使。 因此，生物样品中S1P的定量是在不同体液和器官中的生理和病理生理条件下破译其体内信号传导能力的重要任务。 在该方案中，通过耦合到三重四极杆质谱（LC-MS/MS）的液相色谱法进行S1P的定量。
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