DCTM Protein Assay Kit II
Company: Bio-Rad Laboratories
Catalog#: 5000112
Product evaluation:
In organello Protein Synthesis
Author:
Date:
2014-06-20
[Abstract]  In organello protein synthesis method allows the analysis of mitochondrial translation products. The principle of this method relies on incubation of isolated intact mitochondria with radiolabeled amino acids such as 35S methionine. After protein synthesis, the radiolabeled translation products are subsequently separated by SDS polyacrylamide gel electrophoresis and analysed by autoradiography. For in organello analysis of protein synthesis, the isolated intact ...

Lysosomal Amino Acid Efflux Assay
Author:
Date:
2014-02-20
[Abstract]  As the cellular “recycling” organelle, lysosomes break down proteins into amino acids, which are then transported into cytosol for reuse by various amino acid transporters. The transport rate of an amino acid is presumably regulated by cellular conditions such as organelle pH, membrane potential and metabolic states. Because of their intracellular localization and the relative inaccessibility, lysosomal amino acid transporters have been studied largely via indirect measurements. Using lysosome ...

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