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Author:
Date:
2016-05-05
[Abstract] Mitochondria are essential regulators in not only ATP generation and metabolic reprogramming but also the generation of reactive oxygen species (ROS) in response to pathogenic stimuli. During exposure to environmental stresses including oxidative stress, exercise, cell division and caloric restriction, mitochondria can be divided to increase mitochondrial number, size, and mass. Moreover, mitochondrial biogenesis has a crucial role in the resolution of inflammation through preserving metabolic function. Recently, diverse biochemical methods have been utilized to evaluate activity of mitochondrial biogenesis. In this protocol, we will describe an in vitro assay to measure mitochondrial DNA content and mass. Quantitative real-time PCR analysis for determination of mitochondrial DNA content ...
[摘要] 线粒体是不仅ATP生成和代谢重新编程，而且响应病原性刺激的活性氧（ROS）的生成的必要调节器。在暴露环境应激，包括氧化应激，运动，细胞分裂和热量限制期间，线粒体可以分为增加线粒体数量，大小和质量。此外，线粒体生物发生在通过保护代谢功能解决炎症中具有关键作用。最近，不同的生物化学方法已被用来评估线粒体生物发生的活性。在这个协议，我们将描述一个体外测定来测量线粒体DNA含量和质量。用于测定线粒体DNA含量的定量实时PCR分析是添加流式细胞术或共聚焦显微镜用于评价线粒体质量的有力工具。在一起，这些协议可以提供线粒体研究的重要信息。
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