| Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS
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Author:
Date:
2015-06-20
[Abstract] Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
[摘要] 用热水提取是用于从醇不溶性残余物提取物中回收果胶的最古老和最简单的方法，尽管该方法尚未广泛用于花粉管的细胞壁分析，所述花粉管是用于研究细胞壁的模型。 该方案描述了该方法应用于从6h-旧拟南芥花粉管中提取果胶，然后通过气相色谱质谱法进行糖组成分析。
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| Lysosomal Amino Acid Efflux Assay
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Author:
Date:
2014-02-20
[Abstract] As the cellular “recycling” organelle, lysosomes break down proteins into amino acids, which are then transported into cytosol for reuse by various amino acid transporters. The transport rate of an amino acid is presumably regulated by cellular conditions such as organelle pH, membrane potential and metabolic states. Because of their intracellular localization and the relative inaccessibility, lysosomal amino acid transporters have been studied largely via indirect measurements. Using lysosome purification and 14C-labeled amino acids, this protocol provides a method to measure the efficiency of specific amino acid transporters on lysosomes.
[摘要] 作为细胞"再循环"细胞器，溶酶体将蛋白质分解成氨基酸，然后将其转运到细胞质中以供各种氨基酸转运蛋白重复使用。 氨基酸的转运速率可能通过细胞条件如细胞器pH，膜电位和代谢状态来调节。 由于它们的细胞内定位和相对不可接近性，溶酶体氨基酸转运蛋白主要通过间接测量进行研究。 使用溶酶体纯化和14 C标记的氨基酸，该协议提供了一种测量特定氨基酸转运蛋白在溶酶体上的效率的方法。
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