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Richard-Allan ScientificTM CytosealTM 60 and 280

Richard-Allan ScientificTMCytosealTM 60 and 280

Company: Thermo Fisher Scientific
Catalog#: 8311-4
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Collecting and Fixing Nuclear GFP/RFP in L1 Larva for Imaging
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2012-03-05
[Abstract]  In this protocol, L1 stage larvae are collected that carry nuclear-localized GFP/wCherry reporters. These can be fixed so that the GFP/wCherry maintains nuclear localization and stain nuclei by DAPI. This protocol therefore achieves the collection and fixation of nuclei in worm L1 larvae. [摘要]  在这个协议,收集L1阶段幼虫携带核定位GFP/wCherry记者。 这些可以是固定的,使GFP/wCherry保持核定位和染色核由DAPI。 因此该协议实现了在L1幼虫中核的收集和固定。

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